Research in Immobilized Cofactors of Enzymes Employs Brookhaven’s NanoBrook 90Plus Particle Size Analyzer

Nov 7, 2019
Applications: BiopharmaEnzymes
Instruments: NanoBrook Series
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The Journal of Biotechnology featured a research entitled, “Nanoparticle-Supported Multi-Enzyme Biocatalysis With In Situ Cofactor Regeneration” conducted by Wenfang Liu, Songping Zhang and Ping Wang. The study’s aim is to report on the nature of immobilized cofactors of enzymes used in many biotechnology applications.

The researchers using a Brookhaven NanoBrook 90Plus Particle Size Analyzer for dynamic light scattering analysis of the silica nanoparticles suspended in solution was able to attain silica nanoparticles with effective diameter where enzymes were immobilized. In this study, silica nanoparticle-attached glutamate dehydrogenase (GLDH), lactate dehydrogenase (LDH) and their cofactor NAD(H) were covalently immobilized separately on the surface of silica nanoparticles and were applied successfully for coupled biotransformation reactions with in situ cofactor regeneration. Through this study, it was demonstrated that the use of particle-attached systems will allow easy recovery through methods such as filtration and precipitation, and can be recycled and reused, thus substantially improving the potential of cofactor-dependent biochemical reactions for large-scale industrial bioprocessing applications.

Scientific advances on biotechnology require precision and consistency thus, only reliable instruments are utilized. Brookhaven Instruments offers only high grade particle size analyzers designed to facilitate scientific researches which includes dynamic light scattering analysis sensitive to both small and large particles depending on scattering angle preferred.