Particle Solutions v4 Software for DLS and Zeta Potential Measurements

Advanced Particle Sizing and Zeta Potential Software for Nanoparticle, protein, and polymer characterization.

Particle Solutions Software v4

 

Particle Solutions software is used with the NanoBrook family of Particle Size and Zeta Potential Analyzers and has been updated with a new intuitive user interface and advanced features for those looking to get the most out of their measurements.    

Advanced features include the unique ability for the user to customize the correlator layout for complete control over acquisition of the DLS autocorrelation function and improved data quality algorithms offering end users expert advice in improving their measurement.

Features at a glance 

  • Advanced Correlator Control 
  • Windows® Tablets and Touchscreen Compatible 
  • On-screen guidance for measurement issues 
  • Improved dust rejection algorithm 
  • Multi-peak support for NNLS and Contin 

Overview

The capabilities of the NanoBrook depend heavily on both the instrument control software, Particle Solutions, as well as on Brookhaven’s dedicated high-speed signal processing hardware, especially its TurboCorr correlator. DLS, ELS, PALS, and all other light scattering derived methods involve illumination of the sample using laser light, which is then measured at one of three scattering angles (for ELS and PALS this is always done in forward scatter).  The light scattered is collected from forward, side and backscatter directions with a Single Photon Counting Module based on an Avalanche Photo Diode (APD).  DLS time series data is processed in real time by the correlator, and then sent to Particle Solutions for fitting.   As one of the pioneers in light scattering, Brookhaven Instruments has a long history in the development of the digital correlator. Our current correlator offers 510 hardware channels with 100% efficiency and real-time operation over the entire delay-time range. Particle Solutions is the Windows compatible software used to process data for review. 

 

Particle Solutions v4.0 is the latest version of our powerful instrument control software. Particle Solutions v4.0 incorporates five different standard measurement types based on our core technologies: Dynamic Light Scattering (DLS), Electrophoretic Light Scattering (ELS), Phase Analysis Light Scattering (PALS), Microrheology (μRhe), Absolute Size Exclusion (ASEC), and Debye Plot (DBY) packaged into a single application. This release was upgraded to offer advanced correlator control, improved guidance for measurement issues, compatibility with the Windows operating systems, and well as native support for Windows tablets and touchscreen devices and improved user interface offering easy navigation to commonly used operations. 

 

See the Data That You Want 

The data grid is completely customizable and includes a number of different standard display options. New column layouts can be saved as presets, and easily recalled.  A wide range of column selections are available including count rate, diameter, PDI, and a host of others.

Particle Solutions v4.0 allows the import of data from previous versions of Particle Solutions, as well as from select legacy products, imported data can be given custom labels allowing for easy recall. Filtering options are available to sort data by date, column or tag label. Customized filters can also be saved as presets.

 

 

Customizable Correlator Layout 

A unique feature found in Particle Solutions V4.0 that is not found in other DLS instrumentation is the ability to create custom correlator layouts.

The ability to modify the channel spacing, number of channels, and the first and last delay is especially useful with samples that have a very slow decay rate due to very low mobility whether due to size of the particle or viscosity of the diluent used in sample preparation.

 

 

 

 

 

 

Measurement Review Flexibility 

The multimodal and lognormal distributions can all be viewed with Intensity, Volume, Surface Area, or Number weighting applied.

The “Raw Data” view shows the normalized autocorrelation function, as well as count rate history.  The baseline index and data retention rate is also displayed in this view.  The baseline index is useful in interpreting the quality of the data, whereas the data retention rate can be used to infer the effectiveness of the dust rejection algorithm.  Points discarded by the dust filter are identified in the graph of the count rate history.

Effective Diameter and Poly Dispersity Index (PDI) are displayed along with the measurement options used and any tagging of the user or sample ID for tracking.

 

 

 

 

Help When You Need It

Particle Solutions v4.0 incorporates powerful algorithms to evaluate experimental data for anomalies that will lead to inaccurate interpretation.

Highlighting a measurement group and then selecting the “Health Information” icon on the main screen will provide information related to the measurement and recommendations to improve.

 

 

 

 

 

Zeta Potential Measurements (PALS & ELS) 

Zeta Potential measurement by electrophoretic light scattering (ELS) and Phase Analysis Light Scattering (PALS) can be performed with compatible NanoBrook instrumentation.

The PALS phase modulated method is orders of magnitude more sensitive than standard ELS based on laser doppler electrophoresis.

The increased sensitivity of PALS allows the measurement of particles that have very low electrophoretic mobilities and allows the system to use lower applied voltages for particles in solutions characterized by higher conductivity such as a phosphate buffer solution (PBS). 

 

 

 

 

 

Power of Titration

Particle Solutions v4.0 is compatible with the Brookhaven Instruments BI-ZTU automatic titration system.  The BI-ZTU is helpful when doing studies to determine the effect of pH or to estimate isoelectric point.  The titrator can also be used for additive titrations such as using zeta potential to determine the optimal dosing of a coagulant or flocculent.

The software allows for input of up to four concentrations of an acid/base or other additive chemical being used by the precision controlled dosing pumps. The additive method allows for either linear or geometric dosing.

 

Specifications

Supported Instruments:

  • NanoBrook Omni 
  • NanoBrook 173Plus 
  • NanoBrook 173 
  • NanoBrook 90Plus PALS 
  • NanoBrook 90Plus Zeta 
  • NanoBrook 90Plus 
  • NanoBrook ZetaPALS 
  • NanoBrook ZetaPlus 

 

NOTE: Due to major updates of the software framework, not all instruments are compatible with Particle Solutions v4.0. Contact us to confirm compatibility with your instrument and explore your options. 

 

Recommended Hardware Requirements: 

Below are the recommended minimum requirements for running Particle Solutions v4.0. Your experience may be impacted if your system specifications fall below the minimum.

 

Operating System Windows 10 Pro, Windows 11 Pro, and Windows 10 Enterprise.* 

 

*May require additional setup/configuration. 

CPU Dual Core or better 
RAM 4 GB or better 
Connections NanoBrook Instruments have a USB Type-B port and are supplied with a USB Type-A to Type–B cable for connecting to a computer. USB Type-C devices would require a user-supplied adapter. 

Applications

  • Dynamic Light Scattering (DLS) – A method used to relate Brownian diffusion to effective particle size. In this case temperature is controlled, and the particle is allowed to move freely due to thermal motion, this rapid motion is then analyzed, yielding an equivalent hydrodynamic diameter. Electrophoretic Light Scattering (ELS) – Laser Doppler Electrophoresis is used to determine the zeta potential (ζ) of colloids based on changes to the velocity of the particle in response to an applied DC field.  This method works well for traditional colloidal particles, particularly those with high surface charges, dispersed in low conductivity aqueous diluents.

 

  • Phase Analysis Light Scattering (PALS) – Similar to ELS, PALS measures the motion of particles in response to an electric field, however in contrast to ELS, PALS uses an AC-electric field.  Demodulated phase data is then analyzed to give a mobility which is then is then converted into a Zeta Potential.  This method is capable of detecting extremely low mobilities, making it suitable for a variety of special cases including particles with very low surface charges, samples in non-aqueous solvents with low- or -no formal surface charges, as well for samples dispersed in media with unusually high conductivities.

 

  • Absolute Size Exclusion Chromatography (ASEC) – When coupled with a specialized flow cell the NanoBrook can be used to acquire time dependent DLS data.  The resulting data can be used to produce a live chromatogram, in the event that the flow cell is connected to a GPC system

 

  • DLS Microrheology (μRhe) – Rheological information (elastic modulus — G’, viscous modulus – G’’, and complex viscosity *) can be obtained for various fluids.  In contrast to a standard DLS measurement where the viscosity of the solvent is known, and the size is an unknown, DLS microrheology requires noninteracting probe particles of known size, which are then used to measure the rheological properties of a fluid with an unknown viscosity.

 

  • Surface Zeta Potential (SZP) – A specialized sample cell with an adjustable platform is used to estimate the surface potential of solid substrates and films.  This requires the sample to be immobilized on the sample pedestal in the SZP apparatus, while the entire assembly is immersed in a solution containing a probe particle of known zeta potential. Surface potential is then inferred from perturbations in the zeta potential of the probe particle as it approaches the solid substrate.

 

  • Debye Plot (DBY) – The Debye Plot is a single angle Static Light Scattering (SLS) measurement made using the 90-degree scattering angle.  It can be used to measure the absolute weight averaged molecular weight (Mw) of an unknown sample.  It involves extrapolation with respect to concentration, and typically requires a concentration series of the unknown.  The Debye Approximation works well with small particles (small with respect to radius of gyration, Rg).

Contact Us

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