NanoBrook 173/173Plus

Brookhaven’s NanoBrook 173 instrument is ideal for fast, routine sizing applications in research or quality control.

Description

Reliable and Accurate Performance and Analysis

Brookhaven’s NanoBrook 173 instrument is the go-to-solution for characterization of proteins, antibodies, nanoparticles, macromolecular assemblies and other molecules/particles of small sizes.  The NanoBrook 173 uses backscattering detection for the highest sensitivity and accuracy optimized for molecules and particles less than a few tens of nanometers.

The new NanoBrook 173 protein and particle size analyzer incorporates all you need for fast, routine, sub-micron measurements. Based on the principles of Dynamic Light Scattering (DLS) for particle sizing and distribution, most measurements only take a minute or two.

The NanoBrook 173Plus adds a 90° light scattering angle. Measurements of traditional colloids are usually made at 90° scattering angle due to the unbiased results measured. For nanoparticles and proteins, IgG and peptides, these < 50 nm samples can be measured using the backscattering angle (173°) for best S/N reproducibility of measurements.

Features at a glance

  • Rapid and accurate protein and nanoparticle size distributions
  • Multimodal & unimodal size distribution software
  • ISO 13321 and ISO 22412 compliant results
  • Range > 0.3 nm to 10 µm
  • Customizable reports
  • Ideal for fast, routine sizing applications in research or quality control
  • High power 40 mW diode laser
  • Dynamic light scattering at 173° only (90° available with the NanoBrook 173Plus)
  • Temperature control, -5 °C to 110 °C
  • Compact bench top unit, USB connection

Measurement Principles

Nanoparticles

Dilute suspensions, on the order of 0.0001 to 1.0% v/v are prepared using suitable wetting and/or dispersing agents if required. A small ultrasonicator is sometimes useful in breaking up loosely-held agglomerates. Sample volumes as small as 50 µL are used and the sample is recoverable. Disposable, polystyrene U-shaped cells are used for aqueous and simple alcohol suspensions. Just a few minutes are required for the sample and cell to equilibrate with the actively controlled temperature environment inside the NanoBrook 173.

image of DLS report

 

Speed and low concentration/small size capabilities

The NanoBrook 173 utilizes backscattering to size the proteins/particles by accumulating the correlation function. The smoother the function, the better the results are. For small molecules, the scattered intensity is typically low. The measurement takes longer to get enough statistics from the photons counted to aquire a good results. The backscattering angle makes for a larger scattering volume, measuring more proteins/particles for a quick measurement of their sizes. The advantage of the backscattering is clear for sizes below 10 nm.

Data Presentation

The NanoBrook 173 protein and particle size analyzer offers many choices for data analysis. One choice is the lognormal distribution by: intensity, volume, surface and number. Another choice, the full size distribution (MSD) can be displayed by the same four weighting factors. In the example above, the MSD is presented by volume for lysozyme. The size distribution and cumulative distribution are shown in the table on the right. Lysozyme has a very low polydispersity in this case.

Typical Applications

  • Proteins, IgG, peptides, RNA/DNA
  • Nanoparticles
  • Polymer latexes
  • Pharmaceutical preparations
  • Micelles, Liposomes, Exosomes
  • Oil/Water and Water/Oil emulsions
  • Paints and pigments
  • Colloids
  • Polymers
  • Pigments, inks and toners
  • Cosmetic Formulations
  • Liposomes and biocolloids
  • Emulsions (foodstuffs, cosmetics)
  • Carbon blacks

 

Specifications

Sample Type Most globular proteins, nanoparticles, and small polymers in any non-absorbing liquid
Size Range > 0.3 nm to 10 µm diameter, depending on refractive index and concentration
Sample Cells 50 µL disposable, 1 to 3 mL disposable plastic, glass or quartz cells
Concentration Range 0.1 ppm to 50 mg/mL, depending on refractive index and concentration
Signal Processing Dynamic Light Scattering, DLS
Correlator Brookhaven’s TurboCorr, multitau research grade with 510 hardware channels, covering the equivalent of 1010 linearly-spaced channels, 100% efficiency, real-time operation over the entire delay-time range
Precision ± 1% typically
Temperature Control -5 °C to 110 °C, ± 0.1 °C, active control. No external circulator required.
Condensation Control Purge facility using dry air, nitrogen preferred
Standard Laser 40 mW red diode laser, nominal 640 nm wavelength
Scattering Angle 173°
Data Presentation Average & width, lognormal fit, and multimodal size distribution standard
Compliance ISO 13321 and ISO 22412 compliant results
Power Requirements 100/115/220/240 VAC, 50/60 Hz, 150 Watts
Dimensions 23.3 x 42.7 x 48.1 (HWD)
Weight 15 kg
Environmental Characterisitics Temperature 10 °C to 75 °C

Humidity 0% to 95%, non-condensing

CE Certificate Class I laser product, EN 60825-1:2001 CDRH

Options & Accessories

Options

BI-SFS  Sample filtration system provides and convenient way to clean our light scattering samples, a necessity for samples smaller than 20 nm. Utilizing Teflon tubing and nonreactive components, the BI-SFS is compatible with most smaple filtration needs provided there is at least 5 mL of liquid. For protein and other rare samples, use 20 nm BI-DF20AQ Anatop filters instead.
 BI-IQOQPQ  Qualification for installation, operation and performance.

Accessories

Cells BI-SCP Box of 100 plastic cells with caps, 2.2 to 4.5 mL volume. Use with water.
BI-SM50 Box of 80 plastic cells, disposable, 50 µL volume, sample retrievable, requires BI-SM50A. Use with water
BI-SM50A One sample cell adaptor for use with BI-SM50.
Reference Materials BI-LTX92 Latex reference material for validation of sizing, 15 mL, 92 ± 3 nm
BI-SVK92 Starters validation kit for sizing, 2 mL, 92 ± 3 nm
BI-CC Nitrogein-purge facility for condensation control

Note: current users of the 173 may upgrade to include zeta potential and other angles for particle sizing provided their instrument has the necessary mechanics, optics and hardware. Contact us for more information.

Download Brochure

NanoBrook 173 Brochure PDF

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