Application Library

DLS, Dust, and Baseline Index
Jan 26, 2021

DLS FAQ: The Baseline Index

The baseline index, abbreviated as BI, is a metric that can be used to quantify the impact of dust contamination on a given DLS measurement. Within the vernacular of light scattering, dust is often loosely defined, referring to any minor component too large to properly disperse, moving due to gravity, rather than Brownian motion.

Applications: DLS
Instruments: NanoBrook SeriesParticle ExplorerParticle Solutions
polymer pyramid
Dec 17, 2020

Role of Light Scattering in Production of Performance Polymers and Other Functional Materials

The majority of modern performance polymers are designed to enhance a particular material property. A number of high-profile engineering plastics, resins, and gels are made from these kinds of precursors. Light scattering can be used to solve many of the unique problems encountered when working with unusual functional plastics and associated polymeric precursors.

Applications: ManufacturingMaterialsMolecular WeightPolymers
Instruments: BI-200SMNanoBrook Series
Nov 25, 2020

Automation of pH-titrations using the BI-ZTU

The Brookhaven Instruments Autotitrator (BI-ZTU) provides researchers with a tool to study the effect of pH on zeta potential (ζ).

Applications: TitrationsZeta
Instruments: BI-ZTUNanoBrook Series
pathlength's effect on scattering
Nov 18, 2020

Why are DLS Measurements at High Concentration Difficult?

Despite the versatility of the DLS technique, there are several important considerations that cannot be ignored when using light scattering to characterize high-concentration solutions.

Applications: DLSPolymersSample Prep
Instruments: BI-200SMNanoBrook Series
Figure of Ionic Strength
Nov 12, 2020

Why is Ionic Strength Relevant to DLS Measurements?

The measurement of particle size by dynamic light scattering (DLS) is heavily dependent on the stability of materials being investigated. Interactions between simple, spherical, colloidal particles are most frequently described by a balance between attractive and repulsive interactions.

Applications: ColloidsDLSHigh SaltPolymersZeta
Instruments: BI-200SMNanoBrook Series
Oct 29, 2020

Data analysis and distribution weighting: FAQ for DLS users

Dynamic Light Scattering (DLS) is a popular technique for obtaining size distributions of dispersible colloidal particles as well as other nanomaterials. As with all other light scattering based techniques, the intensity of scattered light is strongly skewed towards larger particles, something that must be considered when analyzing samples with two or more major constituents.

Applications: DLS
Instruments: BI-200SMNanoBrook Series
chromatograms of BSA using light scattering
Sep 2, 2020

Static Light Scattering of Biomacromolecules

Static Light Scattering (SLS) is capable of measuring the average molecular weight of polydisperse materials, as well as of samples with discrete aggregation numbers, such as proteins.

Applications: Molecular WeightProteinsSLS
Instruments: BI-200SMBI-MwA
Useful Measurements of Monoclonal Antibodies (mAbs) with Dynamic Light Scattering
Apr 15, 2020

Guide to Making Useful Measurements of Monoclonal Antibodies (mAbs) with Dynamic Light Scattering

Summary Dynamic light scattering (DLS) is a powerful technique that is amenable to measurement of biological samples, particularly proteins and biopolymers. One of the fastest growing classes of pharmaceutically active biologics are antibodies. These highly versatile proteins are used as the major functional element in immunoassays and other rapid diagnostics, in vaccine production, and as […]

Applications: BiopharmaDLSProteins
Instruments: BI-200SMNanoBrook Series
disc centrifuge sedimentometry diagram illustration
Jan 29, 2020

The X-ray Disc Centrifuge – A Unique Particle Sizing Technique

Summary Disc centrifuges fractionate particles according to both size and density, making them ideal for high-resolution particle sizing. X-Ray based detection further simplifies the process of calculating a particle size distribution from a centrifugation-based instrument, eliminating optical corrections and approximations.  This has been shown to be especially relevant for metal particles and metal oxide, which […]

Applications: Sizing
Instruments: BI-DCPBI-XDC