Application Library

Using Uncertainty Analysis to Optimize GPC System Performance (from Boston ACS meeting, 2010)

Introduction GPC when coupled with Static Light Scattering (SLS) is a fairly modern and powerful technique for determining absolute molecular weight values without the need to rely on traditional column calibration. In GPC-SLS the signal intensity levels rather than elution times are evaluated quantitatively. In addition, unlike in polymer standard calibration, details of the accurate […]

Applications: ChromatographyPolymers
Instruments: BI-DNDCBI-MwA
Posted on: Nov 8, 2019

Molecular Weight of PVP with the BI-MwA

Library Polyvinylpyrrolidone (PVP) a Molecular Weight Determination with the BI-MwA Polyvinylpyrrolidone (PVP) is a polar molecule and is used in a wide variety of applications. For example, since it simply passes through humans, it is a binder in pharmaceutical tablets. Because PVP binds to polar molecules quite well, it is also used as a paper […]

Applications: Polymers
Instruments: BI-MwA
Posted on: Nov 8, 2019

Molecular Weight of PLA with the BI-MwA

Polylactic Acid (Polylactide, PLA) Molecular Weight Determination with the BI-MwA Polylactic acid, (polylactide, PLA) polymers are being used in a wide range of biomedical, commercial, pharmaceutical and academic applications due to their biocompatibility and biodegradability. Applications include stents, sutures and a wide range of uses in food packaging and disposable tableware. However, the molecular weight […]

Applications: Polymers
Instruments: BI-MwA
Posted on: Nov 8, 2019

Advances in Zeta Potential Measurement of Proteins Using the ZetaPALS

Introduction Stability of protein formulations is of critical importance for the rapidly emerging areas of protein therapeutics and vaccine production from recombinant antigens. Zeta potential is the surface charge density at the shear plane, is one of the parameters used in predicting the stability of these preparations. Zeta potential has also been used as an […]

Applications: PolymersZeta
Instruments: NanoBrook Series
Posted on: Nov 8, 2019

How the NanoBrook Instrument can be a Valuable Academic Teaching Tool

Abstract The NanoBrook line of instruments for particle size and zeta potential analysis while a powerful and all-encompassing research aid used throughout the world can also be used for teaching purposes in many undergraduate and non-technical settings where the principles of dynamic light scattering and electrophoretic mobility are important. Introduction Many times throughout the year, […]

Applications: Teaching
Instruments: NanoBrook Series
Posted on: Nov 8, 2019

Guide for Dynamic Light Scattering (DLS) sample preparation

Nomenclature The term solvent refers the pure solvent used to prepare the diluent. Examples of solvents are toluene or water. The diluent may also be referred to as the liquid in DLS textbooks. Diluents are solvent with additives, for example a 10% by weight methanol in water or a 10 mM KNO3 salt in DI […]

Applications: DLSSample PrepTheory
Instruments: NanoBrook Series
Posted on: Nov 8, 2019

Colloidal Polymers

New and exciting applications are tantalizing goals for many researchers and, with uses ranging from new sensitive pregnancy testing kits to military camouflage, polymer chemistry has an intriguing future. The Sussex Polymer Group is based in the School of Chemistry, Physics and Environmental Science at the University of Sussex. It has two full-time faculty members […]

Applications: ColloidsPolymers
Instruments: BI-DCP
Posted on: Nov 8, 2019

Size Measurement of the Proteins RNAse A and Proteinase K and the Effects of Protein Binding

Written by: Douglas Mankiewicz, Bruce B. Weiner Ph.D., Yuanming Zhang Ph.D., and Jean-Luc Brousseau Ph.D. Abstract: Brookhaven Instruments was supplied two protein samples by Northeastern University. The protein size was measured using Brookhaven’s NanoBrook Omni instrument. The proteins were measured individually and then the samples were combined in to one mixture to determine if protein […]

Applications: BiopharmaEnzymes
Instruments: NanoBrook Series
Posted on: Nov 8, 2019

Zooming in on Zeta Potential

The determination of electrophoretic mobility and zeta potential is crucial to understanding the behavior and stability of colloids and suspensions over time. Traditional light scattering methods that are based upon the shifted frequency spectrum are relatively insensitive and can fail when confronted with high viscosity oils, non-polar liquids, high salt concentrations or particles that are […]

Applications: NanoParticlesTitrations
Instruments: BI-SM50NanoBrook Series
Posted on: Nov 8, 2019

Measuring the size and surface charge of exosomes, microvesicles and liposomes

By Bruce B. Weiner, Ph.D. Introduction Vesicles as well as micelles, and microemulsions were samples of interest in the early days of what became dynamic light scattering1. This occurred in the 1970’s even before the invention of the word nanotechnology and the first biotech revolution. Not until the 1990’s, however, were DLS instruments small enough, […]

Applications: Self-assembled
Instruments: NanoBrook Series
Posted on: Nov 8, 2019
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