Application Library

Guide to Making Useful Measurements of Monoclonal Antibodies (mAbs) with Dynamic Light Scattering

Summary Dynamic light scattering (DLS) is a powerful technique that is amenable to measurement of biological samples, particularly proteins and biopolymers. One of the fastest growing classes of pharmaceutically active biologics are antibodies. These highly versatile proteins are used as the major functional element in immunoassays and other rapid diagnostics, in vaccine production, and as […]

Applications: BiopharmaDLSProteins
Instruments: NanoBrook SeriesBI-200SM
Posted on: Apr 15, 2020
disc centrifuge sedimentometry diagram illustration

The X-ray Disc Centrifuge – A Unique Particle Sizing Technique

Summary Disc centrifuges fractionate particles according to both size and density, making them ideal for high-resolution particle sizing. X-Ray based detection further simplifies the process of calculating a particle size distribution from a centrifugation-based instrument, eliminating optical corrections and approximations.  This has been shown to be especially relevant for metal particles and metal oxide, which […]

Applications: Sizing
Instruments: BI-XDCBI-DCP
Posted on: Jan 29, 2020
image of Zeta PALS

ZetaPALS Brochure

Zeta Potential & Particle Size Analyzer For Measurements In: Solvents High Salt Oily or viscous media Near the isoelectric point 1,000x more sensitive than other techniques ZetaPALS: A Whole New Concept For measurements of low mobility, the unique, Brookhaven Instruments ZetaPALS is the answer. There is nothing else like it. With concepts developed at Bristol […]

Applications: Zeta
Instruments: NanoBrook Series
Posted on: Nov 8, 2019

Zeta potential measurement of limestone in seawater by Phase Analysis Light Scattering

Abstract Measurements of limestone in 100% seawater (1.15M ionic strength), 50% seawater, and 25% seawater were conducted on the Brookhaven Instruments NanoBrook ZetaPALS. Below is the procedure for preparing these samples as well as the measurement parameters used in their analyses. Introduction Zeta Potential is relatively easy to measure in cases of high charge and […]

Applications: High SaltOil RecoveryPetrochemicals
Instruments: NanoBrook Series
Posted on: Nov 8, 2019

Using Uncertainty Analysis to Optimize GPC System Performance (from Boston ACS meeting, 2010)

Introduction GPC when coupled with Static Light Scattering (SLS) is a fairly modern and powerful technique for determining absolute molecular weight values without the need to rely on traditional column calibration. In GPC-SLS the signal intensity levels rather than elution times are evaluated quantitatively. In addition, unlike in polymer standard calibration, details of the accurate […]

Applications: ChromatographyPolymers
Instruments: BI-DNDCBI-MwA
Posted on: Nov 8, 2019

Molecular Weight of PVP with the BI-MwA

Library Polyvinylpyrrolidone (PVP) a Molecular Weight Determination with the BI-MwA Polyvinylpyrrolidone (PVP) is a polar molecule and is used in a wide variety of applications. For example, since it simply passes through humans, it is a binder in pharmaceutical tablets. Because PVP binds to polar molecules quite well, it is also used as a paper […]

Applications: Polymers
Instruments: BI-MwA
Posted on: Nov 8, 2019

Molecular Weight of PLA with the BI-MwA

Polylactic Acid (Polylactide, PLA) Molecular Weight Determination with the BI-MwA Polylactic acid, (polylactide, PLA) polymers are being used in a wide range of biomedical, commercial, pharmaceutical and academic applications due to their biocompatibility and biodegradability. Applications include stents, sutures and a wide range of uses in food packaging and disposable tableware. However, the molecular weight […]

Applications: Polymers
Instruments: BI-MwA
Posted on: Nov 8, 2019

Advances in Zeta Potential Measurement of Proteins Using the ZetaPALS

Introduction Stability of protein formulations is of critical importance for the rapidly emerging areas of protein therapeutics and vaccine production from recombinant antigens. Zeta potential is the surface charge density at the shear plane, is one of the parameters used in predicting the stability of these preparations. Zeta potential has also been used as an […]

Applications: PolymersZeta
Instruments: NanoBrook Series
Posted on: Nov 8, 2019