Aggregation propensity is one of the most critical parameters in determining the stability of protein therapeutics, so a fast, low-volume, non-destructive screen is valuable during formulation and stability work. This note shows how Dynamic Light Scattering resolves a monoclonal antibody sample into three populations under a low-pH challenge and serves as an early-warning indicator of […]
Using scattered photon count rates from Dynamic Light Scattering (DLS) measurements to estimate sample concentration is demonstrated in the following work. Here we explore the limits of acceptable sample concentrations, using the NanoBrook’s newly developed DLS with Concentration (DLSC) tool to perform photon counting in addition to DLS.
Measuring size distributions that span nanometers to microns is a unique challenge. Previously, Brookhaven Instruments offered the BI-XDC, a centrifugal instrument that relied on X-Rays to detect sedimenting particles.
The Debye Plot, a common type of single angle static light scattering (SLS) measurement, allows end users to measure the absolute, or mass averaged, molecular weight (Mw) of small polymers and colloids (typically, Rg < 12 nm) without the need for a multi-angle instrument.
Particle sizing is an important technique used for a variety of applications, from drug development to quality control for product consistency. Brookhaven Instruments offers two different techniques for measuring particle size. One is by Dynamic Light Scattering (DLS), and the other by Disc Centrifugation using the DCP. DLS is a core measurement capability of the […]
Cannabidiol, CBD, is a non-psychoactive chemical compound obtained from Cannabis sativa. CBD has many benefits including anti-inflammatory properties and neuropathic pain relief, which may be preferred over other pain-relieving methods due to its non-addictive characteristics. CBD is lipophilic oil and has poor bioavailability due to low solubility in water. A new method for CBD delivery […]
Summary Cannabidiol, CBD, is a non-psychoactive chemical compound obtained from Cannabis sativa, more specifically the hemp plant. CBD has many benefits including anti-inflammatory properties and neuropathic pain relief, which may be preferred over other pain-relieving methods due to its non-addictive characteristics. Furthermore, CBD can be helpful in managing anxiety and mood disorders, improving sleep, and […]
Summary The purpose of this guide is to demonstrate the proper method for preparing a zeta potential validation sample used for checking the performance of the NanoBrook ZetaPlus, ZetaPALS, 90PlusPALS, and Omni instrumentation. The BI-ZR5 validation material is a dark blue, inorganic powder that is insoluble in water. It has a mean particle size of […]
In the previous entry in this series of papers on particle size and size distribution, the main features of the differential and cumulative size distribution functions were defined and discussed using continuous distributions. Continuous distributions can be measured approximately in fractionation experiments† where, for example, data is taken every second as fractionated slices of the […]
Particle size distribution data can be presented numerically (tabular format) or graphically. When presented graphically, there are two types: differential and cumulative. They are related. If one differentiates the cumulative distribution curve, the differential distribution is obtained. If one integrates the differential distribution curve, the cumulative distribution is obtained. Differential Distribution The differential distribution shows […]