Absolute Size Exclusion Chromatography of BSA

Abstract We present results from Bovine Serum Albumin (BSA) measured in an analytical size exclusion chromatography (SEC) system including the Brookhaven BI-MwA static light scattering system. BSA is used ubiquitously as a test protein for concentration and for system calibration and testing. Typical results are presented. Introduction The extraction of insulin from blood plasma was […]

Applications: BiopharmaChromatographyMolecular Weight
Instruments: BI-MwA

Study of Protein Hydrodynamics with Light Scattering: Size and Charge of Lysozyme

By: Yuanmung Zhang Ph.D., Eric Farrell, Douglas Mankiewicz and Zachary Weiner Abstract Effective determination of the size and charge status of biological macromolecules like proteins plays a crucial role in pharmaceutical, clinical and other biotechnological applications. In the present study, dynamic light scattering (DLS) and electrophoretic light scattering (ELS) are employed to characterize the size […]

Applications: Proteins
Instruments: NanoBrook Series

Brookhaven’s BI-200SM used in Caulobacter Crescentus FTSZ Characterization

The American Society for Biochemistry and Molecular Biology presented a study entitled “Characterization of Caulobacter Crescentus FTSZ”. Sen Hou and the other researchers in this study utilized Brookhaven’s BI-200SM Goniometer to obtain effective particle characterization of Caulobacter Crescentus FTSZ through a dynamic light scattering technique. The researchers recognized that self-assembly of the tubulin homolougue FtsZ […]

Applications: Biopharma
Instruments: BI-200SM

Researchers Study CN Micelles with Brookhaven’s NanoBrook 90Plus Particle Size Analyzer

The Journal of Dairy Sciences featured a research study entitled, “Effect of Solvent and Temperature on the Size Distribution of Casein Micelles Measured by Dynamic Light Scattering”, conducted by C.M. Beliciu and C.I. Moraru. The researchers used the NanoBrook 90Plus Particle Size Analyzer to investigate the effect of solvent on the accuracy of casein (CN) […]

Applications: Colloids
Instruments: NanoBrook Series

Zeta Potential in High Ionic Strength Suspension: Sandia National Lab

The Biosystems Research Department at Sandia National Laboratories in California is using a Brookhaven ZetaPALS with MAS for particle sizing and zeta potential determination. Dr Anup Singh, a researcher in the department who uses the instrument frequently, said: “It is a very well engineered bench-top instrument that is simple and easy to use for routine […]

Applications: BiopharmaColloidsDrug Delivery
Instruments: NanoBrook Series

Role of Light Scattering in Production of Performance Polymers and Other Functional Materials

The majority of modern performance polymers are designed to enhance a particular material property. A number of high-profile engineering plastics, resins, and gels are made from these kinds of precursors. Light scattering can be used to solve many of the unique problems encountered when working with unusual functional plastics and associated polymeric precursors.

Applications: ManufacturingMaterialsMolecular WeightPolymers
Instruments: NanoBrook SeriesBI-200SM

Why are DLS Measurements at High Concentration Difficult?

Despite the versatility of the DLS technique, there are several important considerations that cannot be ignored when using light scattering to characterize high-concentration solutions.

Applications: DLSPolymersSample Prep
Instruments: NanoBrook SeriesBI-200SM

Brookhaven Instruments autotitrator, the BI-ZTU

The BI-ZTU is Brookhaven Instruments’ easy-to-use autotitrator which provides research and industrial laboratories with a tool to study the effect of pH or additive concentration on zeta potential (surface charge at the shear plane). This accessory for Brookhaven Instruments’ NanoBrook ZetaPlus or ZetaPALS zeta potential analyzers is an excellent choice for automatically determining the isoelectric point of […]

Applications: TitrationsZeta
Instruments: NanoBrook SeriesBI-ZTU

Brookhaven 90Plus supports vaccine characterization

Research scientists in the Canadian biotechnology company ID Biomedical are using two Brookhaven 90Plus particle size analyzers to provide critical data towards the characterization of the innovative FluINsureT nasal vaccine currently under development. Dr John Williams, Senior Scientist in the Department of Analytical Development explained: “It is vital to know the size of the component […]

Applications: BiopharmaDrug DeliveryZeta
Instruments: NanoBrook Series

NanoBrook 90Plus Particle Size Analyzer Used in Silver Nanoparticles Characterization

The Indian Academy of Sciences’ National Chemical Laboratory conducted a study entitled, “Synthesis of Silver Nanoparticles by Sophorolipids: Effect of Temperature and Sophorolipid Structure on the Size of Particles”. Researchers used Brookhaven’s NanoBrook 90Plus Particle Size Analyzer to determine Dynamic Light Scattering measurements in characterizing silver nanoparticles including its ligand shell. This specific technique was […]

Applications: NanoParticles
Instruments: NanoBrook Series

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