Brookhaven’s NanoBrook ZetaPlus Used in Examining DNA Particles

Nov 7, 2019
Applications: DNANanoParticlesSelf-assembled
Instruments: NanoBrook Series
image of DNA

The Nucleic Acids Research featured a study entitled, “Layer-By-Layer Deposition Of Oppositely Charged Polyelectrolytes On The Surface Of Condensed DNA Particles”. Author Vladimir Trubetskoy, along with his team of researchers, described the layer-by-layer deposition of oppositely charged polyelectrolytes on the surface of condensed DNA particles and the formation of negatively charged particles containing completely condensed DNA. To ensure accurate results from the series of tests conducted, a Brookhaven NanoBrook ZetaPlus particle analyzer was used to obtain zeta potential measurements and particle size.

Particle sizing and zeta potential measurements are important factors that contributed to the success of this study. Data gathered from these measurements allowed the researchers to provide an advanced modeling viral assembly, given that most viruses are negatively charged. The results in this study were beneficial not only to the chromatin and viral assembly but also to the construction of artificial gene delivery vectors.

Zeta potential measurement is used by experts to determine the stability of colloidal dispersions as well as indicate the degree of repulsion between adjacent, similarly charged particles in dispersion. Brookhaven Instruments provides researchers and scientists effective and reliable equipment to get accurate and useful results. Brookhaven Instruments’ NanoBrook ZetaPlus is the simplest and most accurate particle electrophoresis system available. The NanoBrook ZetaPlus is easy-to-use, intuitive to operate, yet offers flexibility and a full range of means for those who wish to tune their experiments to specific circumstances.