Brookhaven’s NanoBrook 90Plus Particle Size Analyzer Aids in Enhancing Intracellular Delivery of Therapeutic Nucleic Acids

Nov 7, 2019
Applications: Drug Delivery
Instruments: NanoBrook Series
image of molecule

A research on biomaterials entitled, “Degradable, Ph-Sensitive, Membrane-Destabilizing, Comb-Like Polymers For Intracellular Delivery Of Nucleic Acids” was featured in Science Direct. The study discussed the synthesis, characterization and membrane-destabilizing activity of a new series of degradable, pH-sensitive, comb-like polymers that could enhance the intracellular delivery of therapeutic nucleic acids.

To facilitate this study, “smart” particles were formed by dissolving pH sensitive polymers in RNase-free water and mixed with anti-GAPDH (Glyceraldehyde 3-phosphate dehydrogenase) short interfering RNA molecules. These “smart” particles then underwent a series of tests, with their size and zeta potential being measured using Brookhaven’s NanoBrook 90Plus Particle Size Analyzer to ensure accurate and consistent results. The results of this study indicated the possibility of these particles to serve as carriers for nucleic acid molecules, therefore making them a potential treatment for cancer, viral infection and cardiovascular and neurodegenerative disease.

The study of small particles is common in the development and research of various applications for biomaterials and biomedical engineering. Because of this, reliable and state-of-the-art equipment is required. Brookhaven Instruments offers accurate, convenient and technologically advanced equipment such as particle size analyzers. Brookhaven’s NanoBrook 90Plus Particle Size Analyzer is a fast, effective and compact instrument, ideal for particle characterization.