Brookhaven’s BI-200SM used in Caulobacter Crescentus FTSZ Characterization

Nov 7, 2019
Applications: Biopharma
Instruments: BI-200SM
image of caulobacter crescentus

The American Society for Biochemistry and Molecular Biology presented a study entitled “Characterization of Caulobacter Crescentus FTSZ”. Sen Hou and the other researchers in this study utilized Brookhaven’s BI-200SM Goniometer to obtain effective particle characterization of Caulobacter Crescentus FTSZ through a dynamic light scattering technique.

The researchers recognized that self-assembly of the tubulin homolougue FtsZ at the mid-cell is a critical step in bacterial cell division. In this study, the researchers introduced dynamic light scattering spectroscopy (DLS) as a new method to studying polymerization kinetics of FtsZ in solutions. Through Brookhaven’s BI-200SM Goniometer, dynamic light scattering measurements were obtained that provided insight into the kinetics and stable length of Caulobacter crescentus FtsZ in vitro. Findings of the study revealed that Caulobacter crescentus FtsZ formed short linear polymers in solutions, with the assembly rate depending on the concentrations of GTP and GDP.

Particle size analysis through the DLS technique determines the size and distribution profile of small particles in suspensions or polymers in solutions, valuable to modern day scientific research. To achieve accurate results for any application, scientists and researchers use only high grade particle size analyzers. Brookhaven Instruments’ BI-200SM Goniometer delivers fast and routine sub-micron measurements.