Brookhaven’s BI-200SM Aids in Research on Local Phospholipid Environment

image of molecules

The Journal of Biological Chemistry featured a research study entitled “The Local Phospholipid Environment Modulates the Activation of Blood Clotting”, conducted by Andrew Shaw and his colleagues. In this study, the researchers made use of Brookhaven’s BI-200SM Goniometer to aid them in their investigation of the local changes in phospholipid bilayer composition and how this modulated the activity of the plasma serine protease, factor VIIa-tissue factor complex.

A dynamic light scattering (DLS) method was performed using Brookhaven’s BI-200SM to analyze the particles used for the purpose of this study. The researchers tested a concept, which stated that the local membrane nanoenvironment surrounding coagulation protease cofactor complexes controlled both their assembly and catalytic efficiencies toward membrane-bound protein substrates. Findings of the study were able to report that full TF-VIIa proteolytic activity required extremely high local concentrations of anionic phospholipids. Additionally, a large pool of membrane-bound fX was not required to support efficient analysis.

Particle size analysis through DLS techniques is used to determine relevant data in scientific research, such as the size and distribution profile of small particles in suspensions or polymers in solutions. To achieve accurate results in any application, scientists and researchers use only high-grade particle size analyzers.

Applications: Biopharma
Instruments: BI-200SM
Posted on: Nov 7, 2019