Insight

Effects of Protein Binding

Size Measurement of the Proteins RNAse A and Proteinase K and the Effects of Protein Binding

Written by: Douglas Mankiewicz, Bruce B. Weiner Ph.D., Yuanming Zhang Ph.D., and Jean-Luc Brousseau Ph.D. Abstract: Brookhaven Instruments was supplied two protein samples by Northeastern University. The protein size was measured using Brookhaven’s NanoBrook Omni instrument. The proteins were measured individually and then the samples were combined in to one mixture to determine if protein […]

Applications: BiopharmaEnzymes
Instruments: NanoBrook Series
Posted on: Nov 8, 2019

Zooming in on Zeta Potential

The determination of electrophoretic mobility and zeta potential is crucial to understanding the behavior and stability of colloids and suspensions over time. Traditional light scattering methods that are based upon the shifted frequency spectrum are relatively insensitive and can fail when confronted with high viscosity oils, non-polar liquids, high salt concentrations or particles that are […]

Applications: NanoParticlesTitrations
Instruments: BI-SM50NanoBrook Series
Posted on: Nov 8, 2019
Exosome Graphs

Measuring the size and surface charge of exosomes, microvesicles and liposomes

By Bruce B. Weiner, Ph.D. Introduction Vesicles as well as micelles, and microemulsions were samples of interest in the early days of what became dynamic light scattering1. This occurred in the 1970’s even before the invention of the word nanotechnology and the first biotech revolution. Not until the 1990’s, however, were DLS instruments small enough, […]

Applications: Self-assembled
Instruments: NanoBrook Series
Posted on: Nov 8, 2019

Nano-Particles at Penn State

A research group in the Department of Chemical Engineering at Pennsylvania State University uses a Brookhaven Instruments ZetaPALS instrument to characterize the zeta potential of titania particles in very low pH environments. Themis Matsoukas, Associate Professor in the department explained: “We work with titania nanoparticles at pH between 0.5 and 2. These particles are formed […]

Applications: NanoParticles
Instruments: NanoBrook Series
Posted on: Nov 8, 2019
Dynamic Light Scattering Banner

What is Dynamic Light Scattering?

Dynamic Light Scattering (DLS) is a measurement technique that provides a fast and simple method for submicron and nanoparticle sizing.

Applications: DLSTheory
Instruments: BI-200SMNanoBrook Series
Posted on: Nov 8, 2019

A Horizontal View of Light Scattering for Characterizing Proteins, Polymers and Nanoparticles

By Bruce B. Weiner Ph.D. Introduction The large number of properties that can be determined using various forms of light scat­tering is impressive: molecular weight, radius of gyration, and second virial coefficient using static light scattering (SLS) on synthetic polymers and natural biopolymers like proteins; diffusion coefficient, hydrodynamic radius, and size distribution using dynamic light […]

Applications: BiopharmaDLSNanoParticlesPolymersSLS
Instruments: BI-200SMNanoBrook Series
Posted on: Nov 8, 2019
Protein Hydrodynamics

Study of Protein Hydrodynamics with Light Scattering: Size and Charge of Lysozyme

By: Yuanmung Zhang Ph.D., Eric Farrell, Douglas Mankiewicz and Zachary Weiner Abstract Effective determination of the size and charge status of biological macromolecules like proteins plays a crucial role in pharmaceutical, clinical and other biotechnological applications. In the present study, dynamic light scattering (DLS) and electrophoretic light scattering (ELS) are employed to characterize the size […]

Applications: Proteins
Instruments: NanoBrook Series
Posted on: Nov 8, 2019
Electrophoretic Light Scattering (ELS) Detection

Free-boundary Electrophoresis Using Electrophoretic Light Scattering (ELS) Detection

Purpose Complementing traditional small-molecular drugs, therapeutic proteins hold a great promise for human therapy to treat hematological and solid tumors, autoimmune, inflammatory, infectious, and cardiovascular diseases. To ensure the safety, quality, integrity, and efficacy of the therapeutic protein, effective characterization techniques are needed to address their inevitably heterogeneity due to the biotechnological manufacture processes. In […]

Applications: Zeta
Instruments: NanoBrook Series
Posted on: Nov 8, 2019
Micelle for Water-Insoluble Drugs

Drug Delivery System: Micelle for Water-Insoluble Drugs

Vitamin E TPGS, d-α-tocopherol polyethylene glycol 1000 succinate, is a surfactant that can be used for drug delivery. TPGS has a molecular structure of a typical amphiphile. Due to its large lipophilic and hydrophilic portions, the Critical Micelle Concentration (CMC) is relatively low. Thus, it is a good emulsifier for a wide range of water-oil […]

Applications: BiopharmaDrug Delivery
Instruments: NanoBrook Series
Posted on: Nov 8, 2019

Dielectric Constant of Hydrocarbon Cleaner

Abstract The Brookhaven Instruments’ BI-870 is used to accurately measure the dielectric constant of a decanamide cleaner. The dielectric value quickly reveals moisture that would lower the quality of the cleaning liquid. Introduction A dielectric material (dielectric for short) is an electrical insulator that can be polarized by an applied electric field. These materials are […]

Applications: Manufacturing
Instruments: BI-870
Posted on: Nov 8, 2019