Study of Protein Hydrodynamics with Light Scattering: Size and Charge of Lysozyme

By: Yuanmung Zhang Ph.D., Eric Farrell, Douglas Mankiewicz and Zachary Weiner Abstract Effective determination of the size and charge status of biological macromolecules like proteins plays a crucial role in pharmaceutical, clinical and other biotechnological applications. In the present study, dynamic light scattering (DLS) and electrophoretic light scattering (ELS) are employed to characterize the size […]

Applications: Proteins
Instruments: NanoBrook Series

Free-boundary Electrophoresis Using Electrophoretic Light Scattering (ELS) Detection

Purpose Complementing traditional small-molecular drugs, therapeutic proteins hold a great promise for human therapy to treat hematological and solid tumors, autoimmune, inflammatory, infectious, and cardiovascular diseases. To ensure the safety, quality, integrity, and efficacy of the therapeutic protein, effective characterization techniques are needed to address their inevitably heterogeneity due to the biotechnological manufacture processes. In […]

Applications: Zeta
Instruments: NanoBrook Series

Drug Delivery System: Micelle for Water-Insoluble Drugs

Vitamin E TPGS, d-α-tocopherol polyethylene glycol 1000 succinate, is a surfactant that can be used for drug delivery. TPGS has a molecular structure of a typical amphiphile. Due to its large lipophilic and hydrophilic portions, the Critical Micelle Concentration (CMC) is relatively low. Thus, it is a good emulsifier for a wide range of water-oil […]

Applications: BiopharmaDrug Delivery
Instruments: NanoBrook Series

Dielectric Constant of Hydrocarbon Cleaner

Abstract The Brookhaven Instruments’ BI-870 is used to accurately measure the dielectric constant of a decanamide cleaner. The dielectric value quickly reveals moisture that would lower the quality of the cleaning liquid. Introduction A dielectric material (dielectric for short) is an electrical insulator that can be polarized by an applied electric field. These materials are […]

Applications: Manufacturing
Instruments: BI-870

Zeta Potential in High Ionic Strength Suspension: Sandia National Lab

The Biosystems Research Department at Sandia National Laboratories in California is using a Brookhaven ZetaPALS with MAS for particle sizing and zeta potential determination. Dr Anup Singh, a researcher in the department who uses the instrument frequently, said: “It is a very well engineered bench-top instrument that is simple and easy to use for routine […]

Applications: BiopharmaColloidsDrug Delivery
Instruments: NanoBrook Series

Zeta Potential and Membrane Fouling; Research at Yale

The Deparment of Chemical and Environmental Engineering, Yale University, USA, carries out advanced research into water purification including studies of natural and engineered aquatic systems. After moving to Yale from UCLA two years ago, the deparment’s Llewellyn West Jones, Professor of Environmental Engineering, Menachem Elimelech, needed a more up-to-date machine to continue his research. Both […]

Applications: ColloidsZeta
Instruments: NanoBrook Series

Use of Zeta Potential Measurements to Characterize Good and Bad Inks

Zeta Potential of Printing Inks Offers Insights into Performance Inks for high-quality applications are frequently applied using a process by which a series of rollers acquire ink selectively according to a pattern formed on their surfaces and then transfer this ink to the printable substrate. This process is controlled by a combination of mechanically and […]

Applications: ColloidsFormulationsZeta
Instruments: NanoBrook Series

Colloidal Stability in Aqueous Suspensions

Why too much dispersant causes problems. Abstract Zeta potential in aqueous suspensions is a function of two variables: charge at the shear plane and free salt ion concentration. [Free here means not attached to the particle surface.] If a dispersant is added to increase the surface charge density (increase stability) and it’s too concentrated, the […]

Applications: ColloidsFormulations
Instruments: NanoBrook Series

BASF chooses Brookhaven again for chemicals research

BASF Agro Research, which develops chemicals for use in agriculture, has purchased a NanoBrook ZetaPALS from Brookhaven Instruments, based on Phase Analysis Light Scattering (PALS), for the determination of zeta potential of colloidal suspensions. Zeta potential is a useful indicator of a formulation’s stability. The new instrument has a particle sizing option based on DLS […]

Applications: Zeta
Instruments: NanoBrook Series

New Analysis of Red Blood Cells (RBC) using the ZetaPALS

By Bruce B. Weiner Ph.D. Introduction: Zeta potential is a useful property to know for colloids, nanoparticles, and proteins. It is a substitute for the otherwise hard to measure surface charge. The zeta potential is not equal to the surface potential but rather it is the electrostatic potential difference between a rotationally averaged point at the […]

Applications: BiopharmaDiagnosticZeta
Instruments: NanoBrook Series