Despite the versatility of the DLS technique, there are several important considerations that cannot be ignored when using light scattering to characterize high-concentration solutions.
The measurement of particle size by dynamic light scattering (DLS) is heavily dependent on the stability of materials being investigated. Interactions between simple, spherical, colloidal particles are most frequently described by a balance between attractive and repulsive interactions.
Dynamic Light Scattering (DLS) is a popular technique for obtaining size distributions of dispersible colloidal particles as well as other nanomaterials. As with all other light scattering based techniques, the intensity of scattered light is strongly skewed towards larger particles, something that must be considered when analyzing samples with two or more major constituents.
Static Light Scattering (SLS) is capable of measuring the average molecular weight of polydisperse materials, as well as of samples with discrete aggregation numbers, such as proteins.
Gel-permeation chromatography (GPC) coupled with Multi-Angle Static Light Scattering (SLS) is a modern and powerful technique for determining absolute molecular weight values without the need to rely on traditional column calibration.
Summary Dynamic light scattering (DLS) is a powerful technique that is amenable to measurement of biological samples, particularly proteins and biopolymers. One of the fastest growing classes of pharmaceutically active biologics are antibodies. These highly versatile proteins are used as the major functional element in immunoassays and other rapid diagnostics, in vaccine production, and as […]
Summary Disc centrifuges fractionate particles according to both size and density, making them ideal for high-resolution particle sizing. X-Ray based detection further simplifies the process of calculating a particle size distribution from a centrifugation-based instrument, eliminating optical corrections and approximations. This has been shown to be especially relevant for metal particles and metal oxide, which […]
Zeta Potential & Particle Size Analyzer For Measurements In: ZetaPALS: A Whole New Concept For measurements of low mobility, the unique, Brookhaven Instruments ZetaPALS is the answer. There is nothing else like it. With concepts developed at Bristol University and Brookhaven Instruments, the ZetaPALS determines zeta potential using Phase Analysis Light Scattering (PALS): a technique […]
Abstract Measurements of limestone in 100% seawater (1.15M ionic strength), 50% seawater, and 25% seawater were conducted on the Brookhaven Instruments NanoBrook ZetaPALS. Below is the procedure for preparing these samples as well as the measurement parameters used in their analyses. Introduction Zeta Potential is relatively easy to measure in cases of high charge and […]
Introduction GPC when coupled with Static Light Scattering (SLS) is a fairly modern and powerful technique for determining absolute molecular weight values without the need to rely on traditional column calibration. In GPC-SLS the signal intensity levels rather than elution times are evaluated quantitatively. In addition, unlike in polymer standard calibration, details of the accurate […]