Advances in Zeta Potential Measurement of Proteins Using the ZetaPALS

Introduction Stability of protein formulations is of critical importance for the rapidly emerging areas of protein therapeutics and vaccine production from recombinant antigens. Zeta potential is the surface charge density at the shear plane, is one of the parameters used in predicting the stability of these preparations. Zeta potential has also been used as an […]

Applications: PolymersZeta
Instruments: NanoBrook Series

How the NanoBrook Instrument can be a Valuable Academic Teaching Tool

Abstract The NanoBrook line of instruments for particle size and zeta potential analysis while a powerful and all-encompassing research aid used throughout the world can also be used for teaching purposes in many undergraduate and non-technical settings where the principles of dynamic light scattering and electrophoretic mobility are important. Introduction Many times throughout the year, […]

Applications: Teaching
Instruments: NanoBrook Series

Guide for Dynamic Light Scattering (DLS) sample preparation

Nomenclature The term solvent refers the pure solvent used to prepare the diluent. Examples of solvents are toluene or water. The diluent may also be referred to as the liquid in DLS textbooks. Diluents are solvent with additives, for example a 10% by weight methanol in water or a 10 mM KNO3 salt in DI […]

Applications: DLSSample PrepTheory
Instruments: NanoBrook Series

Colloidal Polymers

New and exciting applications are tantalizing goals for many researchers and, with uses ranging from new sensitive pregnancy testing kits to military camouflage, polymer chemistry has an intriguing future. The Sussex Polymer Group is based in the School of Chemistry, Physics and Environmental Science at the University of Sussex. It has two full-time faculty members […]

Applications: ColloidsPolymers
Instruments: BI-DCP

Size Measurement of the Proteins RNAse A and Proteinase K and the Effects of Protein Binding

Written by: Douglas Mankiewicz, Bruce B. Weiner Ph.D., Yuanming Zhang Ph.D., and Jean-Luc Brousseau Ph.D. Abstract: Brookhaven Instruments was supplied two protein samples by Northeastern University. The protein size was measured using Brookhaven’s NanoBrook Omni instrument. The proteins were measured individually and then the samples were combined in to one mixture to determine if protein […]

Applications: BiopharmaEnzymes
Instruments: NanoBrook Series

Zooming in on Zeta Potential

The determination of electrophoretic mobility and zeta potential is crucial to understanding the behavior and stability of colloids and suspensions over time. Traditional light scattering methods that are based upon the shifted frequency spectrum are relatively insensitive and can fail when confronted with high viscosity oils, non-polar liquids, high salt concentrations or particles that are […]

Applications: NanoParticlesTitrations
Instruments: NanoBrook SeriesBI-SM50

Measuring the size and surface charge of exosomes, microvesicles and liposomes

By Bruce B. Weiner, Ph.D. Introduction Vesicles as well as micelles, and microemulsions were samples of interest in the early days of what became dynamic light scattering1. This occurred in the 1970’s even before the invention of the word nanotechnology and the first biotech revolution. Not until the 1990’s, however, were DLS instruments small enough, […]

Applications: Self-assembled
Instruments: NanoBrook Series

Nano-Particles at Penn State

A research group in the Department of Chemical Engineering at Pennsylvania State University uses a Brookhaven Instruments ZetaPALS instrument to characterize the zeta potential of titania particles in very low pH environments. Themis Matsoukas, Associate Professor in the department explained: “We work with titania nanoparticles at pH between 0.5 and 2. These particles are formed […]

Applications: NanoParticles
Instruments: NanoBrook Series
Dynamic Light Scattering Banner

What is Dynamic Light Scattering?

Dynamic Light Scattering (DLS) is a measurement technique that provides a fast and simple method for submicron and nanoparticle sizing.

Applications: DLSTheory
Instruments: NanoBrook SeriesBI-200SM

A Horizontal View of Light Scattering for Characterizing Proteins, Polymers and Nanoparticles

By Bruce B. Weiner Ph.D. Introduction The large number of properties that can be determined using various forms of light scat­tering is impressive: molecular weight, radius of gyration, and second virial coefficient using static light scattering (SLS) on synthetic polymers and natural biopolymers like proteins; diffusion coefficient, hydrodynamic radius, and size distribution using dynamic light […]

Applications: BiopharmaDLSNanoParticlesPolymersSLS
Instruments: NanoBrook SeriesBI-200SM