Both disc centrifuges separate particles according to size and density, making these methods ideal for high-resolution particle sizing. The Disc Centrifuge Photosedimentometer (DCP) uses visible light for detection, whereas the X-Ray Disc Centrifuge (XDC) uses a compact X-Ray source. Despite different sources of contrast, the two techniques both operate on similar principles. In both cases, […]
The baseline index, abbreviated as BI, is a metric that can be used to quantify the impact of dust contamination on a given DLS measurement. Within the vernacular of light scattering, dust is often loosely defined, referring to any minor component too large to properly disperse, moving due to gravity, rather than Brownian motion.
The majority of modern performance polymers are designed to enhance a particular material property. A number of high-profile engineering plastics, resins, and gels are made from these kinds of precursors. Light scattering can be used to solve many of the unique problems encountered when working with unusual functional plastics and associated polymeric precursors.
The Brookhaven Instruments Autotitrator (BI-ZTU) provides researchers with a tool to study the effect of pH on zeta potential (ζ).
Despite the versatility of the DLS technique, there are several important considerations that cannot be ignored when using light scattering to characterize high-concentration solutions.
The measurement of particle size by dynamic light scattering (DLS) is heavily dependent on the stability of materials being investigated. Interactions between simple, spherical, colloidal particles are most frequently described by a balance between attractive and repulsive interactions.
Dynamic Light Scattering (DLS) is a popular technique for obtaining size distributions of dispersible colloidal particles as well as other nanomaterials. As with all other light scattering based techniques, the intensity of scattered light is strongly skewed towards larger particles, something that must be considered when analyzing samples with two or more major constituents.
Static Light Scattering (SLS) is capable of measuring the average molecular weight of polydisperse materials, as well as of samples with discrete aggregation numbers, such as proteins.
Gel-permeation chromatography (GPC) coupled with Multi-Angle Static Light Scattering (SLS) is a modern and powerful technique for determining absolute molecular weight values without the need to rely on traditional column calibration.
Summary Dynamic light scattering (DLS) is a powerful technique that is amenable to measurement of biological samples, particularly proteins and biopolymers. One of the fastest growing classes of pharmaceutically active biologics are antibodies. These highly versatile proteins are used as the major functional element in immunoassays and other rapid diagnostics, in vaccine production, and as […]