disc centrifuge sedimentometry diagram illustration

Principles of Operation for Disc Centrifuges

Both disc centrifuges separate particles according to size and density, making these methods ideal for high-resolution particle sizing. The Disc Centrifuge Photosedimentometer (DCP) uses visible light for detection, whereas the X-Ray Disc Centrifuge (XDC) uses a compact X-Ray source. Despite different sources of contrast, the two techniques both operate on similar principles. In both cases, […]

Posted on: Feb 16, 2021
DLS, Dust, and Baseline Index

DLS FAQ: The Baseline Index

The baseline index, abbreviated as BI, is a metric that can be used to quantify the impact of dust contamination on a given DLS measurement. Within the vernacular of light scattering, dust is often loosely defined, referring to any minor component too large to properly disperse, moving due to gravity, rather than Brownian motion.

Applications: DLS
Instruments: NanoBrook SeriesParticle ExplorerParticle Solutions
Posted on: Jan 26, 2021
polymer pyramid

Role of Light Scattering in Production of Performance Polymers and Other Functional Materials

The majority of modern performance polymers are designed to enhance a particular material property. A number of high-profile engineering plastics, resins, and gels are made from these kinds of precursors. Light scattering can be used to solve many of the unique problems encountered when working with unusual functional plastics and associated polymeric precursors.

Applications: ManufacturingMaterialsMolecular WeightPolymers
Instruments: BI-200SMNanoBrook Series
Posted on: Dec 17, 2020

Automation of pH-titrations using the BI-ZTU

The Brookhaven Instruments Autotitrator (BI-ZTU) provides researchers with a tool to study the effect of pH on zeta potential (ζ).

Applications: TitrationsZeta
Instruments: BI-ZTUNanoBrook Series
Posted on: Nov 25, 2020
pathlength's effect on scattering

Why are DLS Measurements at High Concentration Difficult?

Despite the versatility of the DLS technique, there are several important considerations that cannot be ignored when using light scattering to characterize high-concentration solutions.

Applications: DLSPolymersSample Prep
Instruments: BI-200SMNanoBrook Series
Posted on: Nov 18, 2020

Why is Ionic Strength Relevant to DLS Measurements?

The measurement of particle size by dynamic light scattering (DLS) is heavily dependent on the stability of materials being investigated. Interactions between simple, spherical, colloidal particles are most frequently described by a balance between attractive and repulsive interactions.

Applications: ColloidsDLSHigh SaltPolymersZeta
Instruments: BI-200SMNanoBrook Series
Posted on: Nov 12, 2020

Data analysis and distribution weighting: FAQ for DLS users

Dynamic Light Scattering (DLS) is a popular technique for obtaining size distributions of dispersible colloidal particles as well as other nanomaterials. As with all other light scattering based techniques, the intensity of scattered light is strongly skewed towards larger particles, something that must be considered when analyzing samples with two or more major constituents.

Applications: DLS
Instruments: BI-200SMNanoBrook Series
Posted on: Oct 29, 2020

Guide to Making Useful Measurements of Monoclonal Antibodies (mAbs) with Dynamic Light Scattering

Summary Dynamic light scattering (DLS) is a powerful technique that is amenable to measurement of biological samples, particularly proteins and biopolymers. One of the fastest growing classes of pharmaceutically active biologics are antibodies. These highly versatile proteins are used as the major functional element in immunoassays and other rapid diagnostics, in vaccine production, and as […]

Applications: BiopharmaDLSProteins
Instruments: BI-200SMNanoBrook Series
Posted on: Apr 15, 2020